想要獲得高質量蛋白晶體一般需要克服3個難題:1、獲得純度高的,均一的蛋白樣品;2,篩選可結晶的條件;3,優化結晶條件,最后獲得高質量的單晶;其中,篩選可結晶的條件是獲得蛋白質晶體的主要瓶頸之一;
Jena Biosource品牌中有一系列蛋白結晶研究所要用的試劑和耗材,以下給大家介紹一下Jena里面暢銷的蛋白結晶條件篩選試劑盒。
1、JBScreen Basic
JBScreen Basic是基于稀疏矩陣法的蛋白結晶試劑盒,包含了96種篩選條件,整套試劑盒分為4個小規格,每個小規格包含24種條件,24個條件分別密封在螺旋蓋試管中,每管10ml,可以隨時使用。Jena里面的蛋白結晶篩選試劑盒不包含二甲砷酸鹽,用MES進行替代。
二甲砷酸鹽,含砷,是有毒物質
1、 致癌物質;
2、 吸入后對人體有害,刺激眼睛和皮膚,長時間接觸,可導致腎臟和肝臟受損;
3、 能與自由巰基反應,可以與半胱氨酸硫共價結合,所以存在蛋白構象受到嚴重影響的風險;
名稱 |
貨號 |
規格 |
JBScreen Basic 1 |
CS-121 |
24 solutions (10 ml each) |
JBScreen Basic 2 |
CS-122 |
24 solutions (10 ml each) |
JBScreen Basic 3 |
CS-123 |
24 solutions (10 ml each) |
JBScreen Basic 4 |
CS-124 |
24 solutions (10 ml each) |
JBScreen Basic 1 – 4 |
CS-125 |
4 Kits |
JBScreen Basic HTS |
CS-203L |
96 solutions (1.7 ml each) |
2、 JBScreen Classic
這個系列是Jena品牌中最初推出的經典的結晶條件試劑盒,在文獻中的應用以每年30%的速度增長;這個試劑盒包含了240個篩選條件,涵蓋了各種有效沉淀劑和Buffer。整套試劑盒分成了10小規格,每個規格包含了24個條件,如果只想要其中某些條件,是可以單獨購買小規格試劑盒的;
名稱 |
貨號 |
條件數量 |
JBScreen Classic 1 (PEG 400 to 3000 based) |
CS-101L |
24 solutions (10 ml each) |
JBScreen Classic 2 (PEG 4000 based) |
CS-102L |
24 solutions (10 ml each) |
JBScreen Classic 3 (PEG 4000+ based) |
CS-103L |
24 solutions (10 ml each) |
JBScreen Classic 4 (PEG 5000 MME to 8000 based) |
CS-104L |
24 solutions (10 ml each) |
JBScreen Classic 5 (PEG 8000 to 20000 based) |
CS-105L |
24 solutions (10 ml each) |
JBScreen Classic 6 (Ammonium Sulfate based) |
CS-106L |
24 solutions (10 ml each) |
JBScreen Classic 7 (MPD based) |
CS-107L |
24 solutions (10 ml each) |
JBScreen Classic 8 (MPD/Alcohol based) |
CS-108L |
24 solutions (10 ml each) |
JBScreen Classic 9 (Alcohol/Salt based) |
CS-109L |
24 solutions (10 ml each) |
JBScreen Classic 10 (Salt based) |
CS-110L |
24 solutions (10 ml each)
|
JBScreen Classic 1–10 |
CS-114L |
10 Kits |
JBScreen Classic HTS I (PEG based) |
CS-201L |
96 solutions (1.7 ml each) |
JBScreen Classic HTS II (Ammonium Sulfate, MPD, Alcohol and Salt based) |
CS-202L |
96 solutions (1.7 ml each) |
3、JBScreen pentaerythritol
基于兩種新奇的沉淀劑,季戊四醇丙氧基化物和季戊四醇乙基化物,用于生物大分子最初結晶條件的篩選。兩者都包含一個季戊四醇的支鏈型高分子。因此他們不同于傳統的沉淀劑(如MPD和PEG)。另外,季戊四醇聚合物具有冷凍保護劑的功能,蛋白晶體在這些高濃度的沉淀劑中生長,并能夠由晶滴直接冷凍;
名稱 |
貨號 |
規格 |
JBScreen Pentaerythritol 1 (PEP 426 based) |
CS-191 |
24 solutions (10 ml each) |
JBScreen Pentaerythritol 2 (PEP 629 based) |
CS-192 |
24 solutions (10 ml each) |
JBScreen Pentaerythritol 3 (PEE 270 based) |
CS-193 |
24 solutions (10 ml each) |
JBScreen Pentaerythritol 4 (PEE 797 based) |
CS-194 |
24 solutions (10 ml each) |
JBScreen Pentaerythritol 1 – 4 |
CS-195 |
4 Kits |
JBScreen Pentaerythritol HTS |
CS-210L |
96 solutions (1.7 ml each) |
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